Exploring Organ Crosstalk in a Model for Atherosclerosis using Comprehensive Plasma Proteomics
June 25, 2025
Proteomics Stage
- Common animal models for atherosclerosis entail genetic manipulation of lipid metabolizing proteins.
- Proteome profiling of these models has been limited to primarily solid tissues such as diseased liver and aorta.
- To instead profile the plasma proteome of mice as they developed fatty liver and atherosclerosis, a nanoparticle-enrichment strategy was essential.
- Data-independent acquisition mass spectrometry performed on the Orbitrap Astral identified 4,000+ plasma proteins, many of which reflected the proteomic changes during disease progression in the liver and aorta.
- These findings confirm that molecules associated with disease progression in tissues can be measured in circulation, providing the basis for plasma-based assays during pre-clinical intervention studies.
- Therefore, from attending this talk you will discover how plasma proteomics can be used to track disease progression, support preclinical risk assessment and facilitate new opportunities for precision medicine.
