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A Scalable Platform for Single-Cell Co-Profiling of the Transcriptome and Genotype
· scRNAseq is widely used to profile cellular states and CRISPR perturbations, but it only captures transcribed regions and cannot confirm edits at the genomic locus.
· Our solution – a high-throughput DNA plus RNA co-assay based on Semi-Permeable Capsule (SPC) technology, enabling multi-step single-cell processing at scale.
· Whole-transcriptome profiling combined with targeted genotyping by multiplex PCR amplicon sequencing directly maps genotypes to transcriptional states and validates CRISPR edits.
· Scalable workflow supporting user-defined PCR panels, now applied to healthy and malignant human cells to dissect genotype–phenotype relationships at single-cell resolution.
