Automation of the NEBNext Ultra II mRNA workflow for library prep was automated on the SPT Labtech Firefly system. Samples were evaluated on the system by preparing libraries using the workflow automated on the Firefly down to 10ng of input. Results were quantitated by Qbit, and size distributions were measured on a TapeStation. QC Metrics demonstrate that the Firefly is capable of readily generating usable libraries down to the lowest specified total RNA input concentration.  Here we present initial QC data along with descriptions of the kit and the instrument used. These results confirm that NEBNext® Ultra™ II RNA Library Prep Kit are successfully processed across a wide input range on the SPT Labtech Firefly instrument in a core facility environment. 
 
Impact/Innovation Statement: 
This work demonstrates the verified automation of the NEBNext® Ultra II RNA library preparation using the mRNA workflow probing the performance with low input sample mass on the SPT Labtech Firefly platform, expanding the reach of usable sequencing automation options for core facilities and enabling consistent, high-throughput, library production for core facillities.