Arthropods are some of the most ecologically important and speciose members of the animal kingdom, accounting for over half of all described species on Earth. Their evolutionary success is contributed to in large part by the diverse morphology of hardened exoskeletons that are sculpted into a variety of forms and serve a broad array of biological functions. The structuring and formation of the exoskeleton during development is governed by a host of hormonal regulators, and key genes that contribute to the formation of structures that allow species to colonize diverse ecological niches. Among the genes and regulators that govern exoskeletal formation over the course of insect development are the Osiris family of genes: a highly conserved, yet functionally diverse set of genes unique to arthropods, which contribute to the deposition of insect exoskeleton and play fundamental roles in their biological functions. These dosage-sensitive genes are the result of a series of gene duplication events, and genomic analysis by our lab group and others has revealed that member genes in this family are responsible for cell trafficking mechanisms with relevance to development, toxin resistance, reproduction, and other specialized functions. Gene suppression assays, as well as previous RNAseq analysis by our lab have demonstrated the importance of these genes in maintaining insect fitness, and withstanding exposure to environmental stressors. Our results demonstrate the potential for leveraging study of this gene family towards applications in agricultural science and disease vector mitigation.