How can we explore different functions for hormones beyond their classic physiological application? One pleiotropic candidate factor that aligns with this question is Leptin, the human hormone that contributes to the satiety response. The challenge with Leptin is that it may play a role in developing nervous and vascular systems that may enrich our interpretation of Leptin’s physiological role. Recent studies have found LepR on both developing neurons and vascular endothelial cells. Yet the role of LepR in vascular development and potential association in pathology of vascular related disease remains understudied. Our goals were to investigate the spatial distribution of LepR in the developing mouse brain vasculature and to determine the impact of LepR expression in vascular-related diseases using public datasets. Leveraging the public dataset, “Single-cell and Time-series Embryonic Development Endothelial Cell (STED-EC) Atlas”, we determined that LepR is expressed on endothelial cells in major tissues as early as embryonic day E7.5 and E9.0 in the central nervous system. Using single-cell RNASeq and direct immunolabeling, we confirmed that LepR is expressed in the choroid plexus and vasculature of the dorsal anterior forebrain of E13.5 mice, a time of rapid neural tissue expansion and vascular investment. To determine if there is a link between LepR expression and vascular-based diseases, we explored NCBI human transcriptomic datasets. The human LEPR has significantly altered expression in vascular diseases, including hemangioma, retinoblastoma, and ataxia telangiectasia, compared to healthy tissues. These results underscore the importance of using multi-omics databases in exploring links between physiology and disease.