Next-generation sequencing platforms continue to advance, shifting the primary bottleneck in large-scale genomics from sequencing to library preparation. The Twist FlexPrep™ UHT Library Preparation and Hybridization Kit addresses this through two innovations: Normalization by Ligation™ eliminates upfront quantification, and early inline barcoding enables immediate sample pooling after ligation, reducing post-ligation processing steps up to 12-fold and supporting high-throughput 96-plex target capture.
 

Here we demonstrate automated implementation of this workflow on SPT Labtech's firefly®, a liquid handling platform that enables scalable, walkaway library preparation with high reproducibility. Using 384 samples of Bovine gDNA across four input concentrations (30, 60, 90 and 120 ng), we achieved data quality comparable to established genomic methods.
 

Libraries were processed using Element Biosciences Trinity™ chemistry, which integrates target hybridization directly onto the sequencing cartridge, eliminating traditional bead-based capture and reducing workflow complexity, hands-on time, and turnaround.