Background/Aims: Jalview (www.jalview.org) is a free platform for interactive visualisation and analysis of alignments of biomolecular sequences linked to 3D structures and phylogenetic trees. It is free software developed in the Barton Group at the University of Dundee’s School of Life Sciences, and its development is currently supported by The Wellcome Trust. Jalview is widely used in teaching and research and cited over 1000 times a year. It is also a recognized interoperability resource by ELIXIR and ELIXIR-UK. In addition to built-in support for 3D molecular graphics linked to alignments and phylogenetic trees, Jalview interoperates with Pymol and UCSF Chimera/X and provides easy access to databases and webservices provided by the Dundee Resource for Protein Sequence Analysis (DRSASP) and Structure Prediction, and EMBL-EBI. Jalview provides an easy-to-use interface for alignment and analysis of biomolecules linked to genomic Loci via Ensembl and the European Nucleotide Archive, and discovery of experimental and computationally determined 3D structures via the PDBe’s 3D-Beacons network. DRSASP provides a range of services to Jalview users, including JPred, one of the most accurate and widely used tools for protein secondary structure prediction, and a range of third-party tools for biomolecular sequence alignment and analysis via Slivka-bio (https://www.compbio.dundee.ac.uk/slivka). All results can be saved and shared as Jalview projects, which can be viewed online with JalviewJS or the Jalview desktop application. In this poster, we will also highlight the latest developments in Jalview 2.12, which includes support for annotation and database searching with HMMER. Jalview is free open-source software released under the GNU Public License (v3), and has been developed in the UK for over 30 years. See the website for more details and access hands-on exercises and YouTube videos about how to use Jalview. We also welcome students and researchers who’d like to add new capabilities, fix bugs or try out new versions. Say hello at https://discourse.jalview.org/ to keep in contact and find out more.